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10mg/264.00元
50mg/660.00元
100mg/1100.00元
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產品編號 bs-0295P
英文名稱 Rabbit IgG
中文名稱 兔IgG(親和層析純化)
別    名 Rabbit Immunoglobulin G; Normal Rabbit IgG; Native Rabbit IgG  
Specific References  (47)     |     bs-0295P has been referenced in 47 publications.
[IF=1.08] Lee, Ju Yeon, et al. "Molecular cloning and expression analysis of pig CD7." Veterinary Research Communications (2014): 1-7.  Pig.  
[IF=.73] Wang, Zhaojun, et al. "Vitamin K2 can suppress the expression of Toll-like receptor 2 (TLR2) and TLR4, and inhibit calcification of aortic intima in ApoE-/-mice as well as smooth muscle cells." Vascular (2017): 1708538117713395.  IHC-P ;  Mouse.  
[IF=0] Miyabe, Yoshishige, et al. "Complement C5a receptor is the key initiator of neutrophil adhesion igniting immune complex–induced arthritis." Science Immunology 2.7 (2017): eaaj2195.  IHC-P ;  Mouse.  
[IF=0] Hao, Yingying, et al. "c-Fos mediates α1, 2-fucosyltransferase 1 and Lewis y expression in response to TGF-β1 in ovarian cancer." Oncology Reports.  IHC-P ;  
[IF=0] Deng et al. Expression and clinical significance of annexin A2 and human epididymis protein 4 in endometrial carcinoma. (2015) J.Exp.Clin.Cancer.Re. 34:96  IHC-P ;  Human.  
[IF=0] Chen X et al.Dual-digital encoded suspension array based on Raman spectroscopy and laser induced breakdown spectroscopy for multiplexed biodetection.(2019)Sensors and Actuators B: Chemical, 282, 457–468.  other ;  Rabbit .  
[IF=0] Halbgebauer R. Early changes in immune, coagulation, and organ function after clinical and experimental polytrauma. 2018  FCM ;  
[IF=] T H et al. Layer-by-layer self-assembly of MoS2/PDDA hybrid film in microfluidic chips for ultrasensitive electrochemical immunosensing of alpha-fetoprotein. Microchemical Journal,(2020) 158, 105209.  Other ;  
[IF=0] Du YQ et al. A study of polyethylene glycol backfilling for enhancing target recognition using QCM-D and DPI.(2018) Journal of Materials Chemistry B.   CLSM ;  
[IF=12.11] Shang, Luoran, et al. "Photonic Crystal Microbubbles as Suspension Barcodes." Journal of the American Chemical Society (2016).  other ;  
[IF=11.56] Shen et al. Brain-specific deletion of histone variant H2A.z results in cortical neurogenesis defects and neurodevelopmental disorder. (2018) Nucleic.Acids.Res. 46:2290-2307  IF ;  Mouse.  
[IF=10.1] Shen, Tianjin, et al. "Brain-specific deletion of histone variant H2A. z results in cortical neurogenesis defects and neurodevelopmental disorder." Nucleic Acids Research.  IHC ;  
[IF=9.66] Zhao, Shuai, et al. "Kinetic and high-throughput profiling of epigenetic interactions by 3D-carbene chip-based surface plasmon resonance imaging technology." Proceedings of the National Academy of Sciences (2017): 201704155.  other ;  
[IF=9.518] He Q et al. Spectral-optical-tweezer-assisted fluorescence multiplexing system for QDs-encoded bead-array bioassay. Biosens Bioelectron. 2019 Mar 15;129:107-117.  Other ;  -.  
[IF=8.37] Yang, Ting, and Jiang Jiang. "Embedding Raman Tags between Au Nanostar@ Nanoshell for Multiplex Immunosensing." Small (2016).  other ;  
[IF=8.22] Xia, Wenlong, and Jianwei Jiao. "Histone variant H3. 3 orchestrates neural stem cell differentiation in the developing brain." Cell Death & Differentiation (2017).  
[IF=7.78] Zhuang et al. Human epididymis protein 4 in association with Annexin II promotes invasion and metastasis of ovarian cancer cells. (2014) Mol.Cancer. 13:243  Control,IHC ;  Human.  
[IF=7.62] Forsberg et al. CO2-evoked release of PGE2 modulates sighs and inspiration as demonstrated in brainstem organotypic culture. (2016) Elife. 5   IHC ;  Mouse.  
[IF=6.641] Zhao J et al. A SERS-based immunoassay for the detection of α-fetoprotein using AuNS@ Ag@ SiO2 core-shell nanostars. Journal of Materials Chemistry C. 2019.  SERS-based sandwich immunoassay ;  
[IF=5.9] Braun, Molly, et al. "Selective activation of cannabinoid receptor-2 reduces neuroinflammation after traumatic brain injury via alternative macrophage polarization." Brain, Behavior, and Immunity (2017).  
[IF=5.69] Wang, Huimin, et al. "Annexin A4 fucosylation enhances its interaction with the NF-kB p50 and promotes tumor progression of ovarian clear cell carcinoma." Oncotarget (2016).  IP ;  Human.  
[IF=5.04] Benesch, Matthew GK, et al. "Tumor-induced inflammation in mammary adipose tissue stimulates a vicious cycle of autotaxin expression and breast cancer progression." The FASEB Journal 29.9 (2015): 3990-4000.  IHC-P ;  Human.  
[IF=5.01] Ma, Hongwei, et al. "Surface initiated polymerization from substrates of low initiator density and its applications in biosensors." ACS applied materials & interfaces 2.11 (2010): 3223-3230.  
[IF=5.01] Christensen, Anders, et al. "uPAR-targeted optical near-infrared (NIR) fluorescence imaging and PET for image-guided surgery in head and neck cancer: proof-of-concept in orthotopic xenograft model." Oncotarget.  FCM ;  Human.  
[IF=5.008] Cao et al. Leptin promotes migration and invasion of breast cancer cells by stimulating IL-8 production in M2 macrophages. (2016) Oncotarge. 7:65441-65453  IHC ;  Mouse.  
[IF=4.8] Benesch, Matthew GK, et al. "Autotaxin is an inflammatory mediator and therapeutic target in thyroid cancer." Endocrine-related cancer 22.4 (2015): 593-607.  IHC-P ;  Human.  
[IF=4.76] Jiang, Wen-Shuai, et al. "Reduced graphene oxide-based optical sensor for detecting specific protein." Sensors and Actuators B: Chemical (2017).  other ;  
[IF=4.439] Zhou W et al. Controlled Hierarchical Architecture in Poly [oligo (ethylene glycol) methacrylate-b-glycidyl methacrylate] Brushes for Enhanced Label-free Biosensing.Applied Surface Science. 450;(2018) 236–243.  other ;  
[IF=3.909] Zhu B et al. The hepatoprotective effect of polysaccharides from Pleurotus ostreatus on carbon tetrachloride-induced acute liver injury rats.Int J Biol Macromol. 2019 Jun 15;131:1-9.   Other ;  
[IF=3.84] Wu, Yuanzi, Hongwei Ma, and Dayong Gu. "Quartz Crystal Microbalance as a tool for biomolecular interaction study." RSC Advances (2015).  other ;  
[IF=3.72] Wu, Qiong, et al. "A highly sensitive SPR biosensor based on a graphene oxide sheet modified with gold bipyramids, and its application to an immunoassay for rabbit IgG." Microchimica Acta (2015): 1-8.  other ;  
[IF=3.571] Peng T et al. The interaction of LOXL2 with GATA6 induces VEGFA expression and angiogenesis in cholangiocarcinoma. Int J Oncol. 2019 Jul 15.   CoIP ;  
[IF=3.561] Chen X et al. Dual-wavelength?digital?holographic?phase?and?fluorescence?microscopy?combining?with?Ramanspectroscopy?for?micro-quartz?pieces-based?dual-channel?encoded?suspension array. Opt Express. 2019 Feb 4;27(3):1894-1910.  Other ;  -.  
[IF=3.55] Huang, Mo, et al. "Solidified liquid layer model makes quartz crystal microbalance a convenient molecular ruler." Colloids and Surfaces B: Biointerfaces 85.1 (2011): 92-96.  
[IF=3.36] Iriyama et al. Direct effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes. (2016) Cancer.Med. 5:3223-3234  Control,FC/FACS ;  Human.  
[IF=3.286] Wang B et al. Gold-nanorod-enhanced Raman spectroscopy encoded micro-quartz pieces for the multiplex detection of biomolecules. Anal Bioanal Chem. 2019 Jul 6.  Other ;  
[IF=3.23] Chenna Narendra S, Chalise JP, Magnusson M, Uppugunduri S (2015) Local but Not Systemic Administration of Uridine Prevents Development of Antigen-Induced Arthritis. PLoS ONE 10(10): e0141863  IHC-P ;  Mouse.  
[IF=3.14] Zhuang, Huiyu, et al. "Overexpression of Lewis y antigen promotes human epididymis protein 4-mediated invasion and metastasis of ovarian cancer cells." Biochimie (2014).  
[IF=3.118] Qu S et al. The effects of resiquimod in an ovalbumin-induced allergic rhinitis model.Int Immunopharmacol. 2018 Jun;59:233-242.   IF ;  Mouse.  
[IF=3] Zhang, Jie, et al. "Role of PTX3 in corneal epithelial innate immunity against Aspergillus fumigatus infection." Experimental Eye Research (2016).  IHC-P ;  
[IF=2.939] Wei S et al. Overexpression of Toll-like receptor 4 enhances LPS-induced inflammatory response and inhibits Salmonella Typhimurium growth in ovine macrophages.(2018) Eur J Cell Biol.   FCM ;  Rabbit.  
[IF=2.72] Wang et al. Blocking AGE-RAGE Signaling Improved Functional Disorders of Macrophages in Diabetic Wound. (2017) J.Diabetes.Res. 2017:1428537  other ;  Mouse.  
[IF=2.55] Chen, Baiwen, et al. "Effects of 1, 25-dihydroxyvitamin D3 in an ovalbumin-induced allergic rhinitis model." International Immunopharmacology 47 (2017): 182-189.  IHC-F ;  Mouse.  
[IF=2.5] Fan, Kequan, et al. "A SERS-based microfluidic immunoassay using an in-situ synthesized gold substrate." Third International Symposium on Laser Interaction with Matter. International Society for Optics and Photonics, 2015.  other ;  
[IF=2.03] Jiang, Wen-Shuai, et al. "Microshell Arrays Enhanced Sensitivity in Detection of Specific Antibody for Reduced Graphene Oxide Optical Sensor." Sensors 17.2 (2017): 221.  other ;  
[IF=1.75] Zhou, S. M., et al. "Natural IgG antibodies in normal rabbit serum are involved in killing of the ompP2 mutant of Haemophilus parasuis SC096 strain via the classical complement pathway." The Veterinary Journal 196.1 (2013): 111-113.  other ;  Others.  
[IF=1.588] Tanaka M et al. Effect of mirabegron on tight junction molecules in primary cultured rat Sertoli cells. Andrologia. 2019 Jan 31:e13241.   ICC ;  
性    狀 Lyophilized powder
濃    度 N/A
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
產品介紹 Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

產品圖片
Blank control:NIH/3T3.
Isotype Control:Rabbit IgG(bs-0295P)
Dilution: 1ug/Test;
Primary Antibody (green line): Rabbit Anti- beta-Actin antibody (bs- 0061R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Isotype control antibody (orange line) was rabbit IgG . The secondary antibody(blue line) used for 40 min at room temperature.Acquisition of 20,000 events was performed.
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